METHOD FOR PREPARING MELIODOSIS PATHOGEN CAPSULE ANTIGEN ELICITING ANTI-PHAGOCYTIC ACTIVITY

摘要

FIELD: medicine, infectious diseases.;SUBSTANCE: invention relates to a method for preparing capsule antigen of meliodosis pathogen eliciting anti-phagocytic activity. Method involves culturing the strain B. pseudomallei, inactivation, isolation of glycoprotein by gel-filtration and ion-exchange chromatography methods resulting to preparing glycoprotein with molecular mass 200 kDA consisting of 90% of carbohydrates, 10% of proteins and comprising 2.17% of nitrogen, 46.4% of carbon and 8.09% of hydrogen. Advantage of method involves preparing the purified glycoprotein eliciting anti-phagocytic activity.;EFFECT: improved method for preparing, valuable properties of antigen.;2 dwg, 5 ex