STRAIN OF CULTURED MURINE HYBRIDOMA I4 CELLS USED FOR PREPARING MONOCLONAL ANTIBODIES RAISED TO ANTIGEN OF NUCLEAR CELL MICROTUBULES

摘要

FIELD: biology, biotechnology, medicine.;SUBSTANCE: strain of murine hybridoma cells is prepared by fusion of murine splenocytes immunized with cell lysates of lymphoblastoid line RAMOS with cells of murine myeloma. Hybridoma secrets monoclonal antibodies directed to antigen of molecular mass 110 kDa located on microtubules in cell cytoplasm and detected in nuclear cells of different species. Using the invention allows studying pathogenesis of cell dividing and state of mitotic activity of health and malignant cells, and evaluation of anti-mitotic (anti-tumor) effect of different chemopreparations and substances. Invention can be used for identifying phase of mitotic activity of cells.;EFFECT: valuable properties of strain.;2 dwg