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Leu-HIRUDINE PRECURSOR AND METHOD FOR PREPARING Leu-HIRUDINE
Leu-HIRUDINE PRECURSOR AND METHOD FOR PREPARING Leu-HIRUDINE
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机译:亮氨酸-亮氨酸前体和制备亮氨酸-亮氨酸的方法
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摘要
FIELD: biotechnology, microbiology, medicine.;SUBSTANCE: method involves selection of signal sequence suitable for the effective expression of Leu-hirudine in E. coli cells by the polymerase chain reaction-screening method. Method involves construction of a protein as a precursor of hirudine based on the selected signal sequence of surface membrane protein from Serratia marcescens, oprF protein from Pseudomonas fluorescens or fumarate reductase from Shewanella putrifaciens by joining the Leu-hirudine amino acid sequence with C-end of selected signal sequence. Prepared precursor of Leu-hirudine is used in a method for preparing Leu-hirudine. Invention provides preparing Leu-hirudine by the direct secretion in E. coli cells with the high yield. Invention can be used in preparing the hirudine precursor.;EFFECT: improved preparing method.;4 cl, 1 dwg, 2 tbl, 12 ex
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机译:领域:方法:该方法涉及通过聚合酶链反应筛选方法选择适合于在大肠杆菌细胞中有效表达亮丙氨酸的信号序列。方法涉及通过将Leu-hirudine氨基酸序列与C-末端的C-末端连接,根据选定的粘质沙雷氏菌表面膜蛋白,荧光假单胞菌的oprF蛋白或恶臭假单胞菌的富马酸酯还原酶的信号序列,构建蛋白作为水rud素的前体。选择的信号序列。制备的Leu-hirudine的前体用于制备Leu-hirudine的方法中。本发明提供了通过在大肠杆菌细胞中直接分泌以高产率制备亮丙氨酸的方法。本发明可用于制备水rud素前体。效果:改进的制备方法。4 cl,1 dwg,2 tbl,12 ex
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