Sensory membrane - osmo meters and osmotic measuring method for quantitative determination of low molecular weight affinity ligand

摘要

Sensory membrane - osmo meters, in the case of the– an affinity receptor (1) for an analyte (2) and a with the analyte (2) of competing affinity ligand of the affinity receptor, of the competition ligand (3), is in a sensory liquid phase (4) are present which, by a semipermeable membrane (5) against a second liquid phase, the medium phase (6), is delimited,– the membrane for the analyte (2) is slightly permeable and for the affinity receptor (1) as well as the competition ligands (3) is not permeable,– at least one of the not permeable affinity binding partner (1 or 3) according to the resolution of the affinity binding to the other party is hydraulically on the membrane on the basis of his osmotic partial pressure is effective,– a measuring device (7) for detection of a on the diaphragm between the two liquid phases (4 and 6) or a pressure difference occurring by the diaphragm between the two liquid phases occurring volume flow is present and– the concentration of the two not permeable affinity binding partner (1 and 3) for the detection of the as-..