Coating reaction mixture containing target molecules onto microarray of interceptor molecules, provides molecules with protective groups which are removed after coating

摘要

Coating reaction mixture containing target- or interceptor molecules are provided with protective groups. The mixture is coated, to cover the microarray completely. Protection is then removed from the molecules provided with protective groups. Interceptor- (3) and target molecules (7) comprise nucleic acids. These and the protective groups (4), comprise nucleic acids. The interceptor molecules form interceptor-target-hybrids with target molecules, having melting points in a temperature range Tm1. The interceptor- or target molecules used, form protective hybrids with protective groups, having a melting points in a temperature range Tm2. The Tm2 range, lies below the Tm1 range. The interval between ranges is 0-40 [deg] C. The first stage is carried out at T1 below Tm2 and the third stage at a temperature T2 greater than Tm2. Interceptor molecules, target molecules and protective groups are proteins, modified proteins, DNA, cDNA, RNA, tRNA, LNA, PNA, aRNA or combinations of them. The protective groups are bound covalently. The protective groups attach themselves to the interceptor- or target molecules. The first stage is accomplished by intentional alteration of the secondary or tertiary structure of interceptor- and/or target molecules. This is canceled when removing the protection. These stages are accomplished by altering physical magnitudes and/or chemical reaction. An independent claim is included for the corresponding microarray.