Method for the investigation of cytosine - methylations in dna with the aid of dna - repair enzymes

摘要

The following invention relates to a process for the investigation of cytosine - methylations with the aid of dna - repair enzymes. In this case, first, the dna is converted so that unmethylated cytosines will be converted to uracil, while 5 - methylcytosine remains unchanged. Subsequently, the dna hybridizes to oligonucleotides, wherein, depending on the methylation status of the dna hybrids with or without base mismatches may also be formed. Thereafter, the error by repair enzymes mismatched hybrids cut. Via different paths can then be determined, the methylation status of the dna. The method according to the invention is particularly suitable for the diagnosis or prognosis of cancer diseases and other by means of a change of the methylation state of an associated diseases, as well as for predicting undesirable side effects.