Identifying and quantifying tumor-associated peptides by chemically identically modifying peptides to generate different physical characteristics, useful for treating tumorous and/or adenomatous diseases

摘要

Identifying and quantifying tumor-associated peptides comprises chemically identically modifying peptides from samples in order to generate different physical characteristics in the peptides from the different samples. The method of identifying and quantifying tumor-associated peptides as cited above further comprises providing a first sample of tissue or cells, providing a second sample of tissue or cells with the identical amount by weight or cellular count as the first sample, obtaining peptides from the first and the second sample, separately, chemically identically modifying the peptides from both samples in order to generate different physical characteristics in the peptides from the different samples, mixing of the so modified peptides from both samples, determining the amino acid sequences of the peptides, and determining the relative amount ratios of peptides from both samples having identical sequences, based on the different physical characteristics. Independent claims are also included for the following: (1) a method for producing a peptide, where a peptide is identified by the method cited above, and the identified peptide is synthesized chemically, in vitroor in vivo; (2) a peptide identified by the method cited above and/or produced by the method of (2); (3) a tumor-associated peptide having any of 36 fully defined sequences of 8, 9 or 10 amino acids (SEQ ID NO: 1-36) from the accompanying sequence protocol, where the peptide has the ability to bind to a molecule of the human major histocompatibility complex (MHC) class-I; (4) a pharmaceutical composition containing one or several peptides of (2) and (3); (5) a nucleic acid molecule encoding the peptide of (2) or (3); (6) a vector comprising the nucleic acid molecule of (5); (7) a cell that was genetically modified with the aid of the nucleic acid molecule of (5) or with the vector of (6) to express a peptide of (2) or (3); (8) a method for producing a vaccine, comprising performing the method cited above, producing the identified peptides, and formulating the produced peptides into the vaccine; (9) a diagnostic method, where the method cited above is performed, and the presence and/or the amount ratio of a peptide is used as a diagnostic marker; (10) a method for treatment of a pathological condition, where an immune response against a protein of interest is triggered, comprising administering at least one of the peptides of (2) and (3); and (11) an electronic storage medium containing the amino acid sequence of at least one of the peptides of (2) or (3) and/or the nucleic acid sequence of (5). ACTIVITY : Cytostatic. No biological data given. MECHANISM OF ACTION : Vaccine; Gene-Therapy.