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CULTURAL INDUSTRIAL PROMOTOR QUENCES

机译:文化产业促进者数量

摘要

This invention discloses a novel cold-inducible promoter which induces gene expression at low temperatures in potato tubers but which is scarcely induced in organs other than tuber or at normal temperature, which induces gene expression for a long time not less than five months. The promoters of this invention are the DNA sequence having a nucleotide sequence from first to 3546th nucleotide in the nucleotide sequence shown in SEQ ID. No. 1, or a part thereof having a cold-inducible promoter activity, or a DNA sequence having the same nucleotide sequence as said DNA sequences except that one or more nucleotides are deleted or substituted, or one or more nucleotides are inserted or added, which DNA sequence has a cold-inducible promoter activity and the DNA sequence having a nucleotide sequence from first to 4120th nucleotide in the nucleotide sequence shown in SEQ ID. No. 2, or a part thereof having a cold-inducible promoter activity, or a DNA sequence having the same nucleotide sequence as the said DNA sequences except that one or more nucleotides are deleted or substituted, or one or more nucleotides are inserted or added, which DNA sequence has a cold-inducible promoter activity.
机译:本发明公开了一种新颖的冷诱导型启动子,其在马铃薯块茎中在低温下诱导基因表达,但在块茎以外的其他器官中或在常温下几乎不被诱导,其诱导基因表达的时间不少于五个月。本发明的启动子是在SEQ ID NO:1所示的核苷酸序列中具有第一个至第3546个核苷酸的核苷酸序列的DNA序列。 No.1,或其具有冷诱导启动子活性的部分,或具有与所述DNA序列相同的核苷酸序列的DNA序列,除了一个或多个核苷酸被缺失或取代,或一个或多个核苷酸被插入或添加,该DNA序列具有冷诱导的启动子活性,该DNA序列具有SEQ ID NO:1所示核苷酸序列中从第一个核苷酸到第4120个核苷酸的核苷酸序列。 2号,或其具有冷诱导启动子活性的部分,或具有与所述DNA序列相同的核苷酸序列的DNA序列,除了一个或多个核苷酸被缺失或取代,或一个或多个核苷酸被插入或添加,该DNA序列具有冷诱导的启动子活性。

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