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Improved mutants of (2,5 tct) reductase a
Improved mutants of (2,5 tct) reductase a
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机译:(2,5 tct)还原酶a的改良突变体
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摘要
Mutants of 2,5-diketo-D-gluconic acid reductase A and B, enzymes used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C), are prepared by site-directed mutagenesis. These mutants may exhibit one or more of the following characteristics: improved temperature stability, increased resistance to substrate inhibition, increased turnover of the substrate by the enzyme and increased affinity for the substrate.
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