Measurement/characterization of a biomass line in fermentation of bacteria comprises measurement of capacitance/optical density of medium for plurality of frequency and treatment of signals of capacitance/optic density measurement

摘要

Measurement and characterization in a biomass line in a process of fermentation of lactic bacteria in a suspension medium, comprising a measurement of the capacitance C of the medium for several frequencies, a measurement of the optical density of the medium for the frequencies and treatment in actual environment of the signals of capacitance and optic density measurement for determining the variables, is new. Measurement and characterization in a biomass line in a process of fermentation of lactic bacteria in a suspension medium, comprising a measurement of the capacitance C of the medium for several frequencies, a measurement of the optical density of the medium for the frequencies and treatment in actual environment of the signals of capacitance and optic density measurement for determining at least one of the three variables: concentration of biomass X, size of the lactic bacteria (phi ) and physiological state (psi ) of the lactic bacteria. Independent claims are also included for: (1) device for measurement and characterization of a biomass line in a fermentation process of lactic bacteria in the suspension medium and implementing the procedure, comprising: (a) means to measure the capacitance C of the medium for several frequencies; (b) means to measure the optical density of the medium and means to treat in actual environment of the signals of capacitance and optic density measurement for determining at least one of the three variables: concentration of biomass X, size of the lactic bacteria and physiological state of the lactic bacteria; and (c) means to treat the measured signals of capacitance and of density; and (2) piloting of a fermentation system of lactic bacteria regulated by adjustment of active parameters to obtain a biomass satisfying at least a target criteria determined in terms of cell size or physiological activity, comprising calculation of at least one of these active parameters from at least one parameter of physical state measured by means of the measuring device.