METHOD FOR RAPIDLY AND HISTOCHEMICALLY STAINING ACETYLCHOLINE ESTERASE

摘要

PROBLEM TO BE SOLVED: To provide a method for rapidly and histochemically staining acetylcholine esterase.;SOLUTION: The method for rapidly and histochemically staining the acetylcholine esterase comprises adding a solution A containing iodized acetylcholine, a citric acid salt and copper sulfate, and a solution B containing potassium ferricyanide to a frozen tissue fragment having 3-6 μm thickness, incubating the resultant fragment at 10-30°C for 4-6 min, adding a diaminobenzidine salt and hydrogen peroxide to the incubated product, further carrying out the incubation at 10-30°C for 1-2 min, and washing the product with water.;COPYRIGHT: (C)2006,JPO&NCIPI