METHOD FOR DETERMINING BASE SEQUENCE OF PROBE NUCLEIC ACID BASED ON HIGHER-ORDER STRUCTURE, AND DNA MICROARRAY

摘要

PROBLEM TO BE SOLVED: To provide a method for designing an oligonucleotide having the heightened certainty of formation of a double-stranded chain, and heightened sequence specificity by considering the higher-order structure such as the secondary structure of a target gene, and a DNA microarray mounting the oligonucleotide as a probe; and to improve the sensitivity, the repeatability and the ability for identifying the target gene, of the DNA microarray.;SOLUTION: The probe is designed by predicting a stem region for forming the double-stranded chain by autocompensation properties, and a loop region of a single-stranded chain from the higher-order structure such as the secondary structure of the target gene sequence, and evaluating the sequence similarity at the vicinity of the loop region and an experimental condition to narrow down the region suitable for hybridization of the oligonucleotide. The sequence of the loop region shown in the figure is sometimes different in a family sequence of a mRNA, and can be identified. The hybridizing ability with the probe of a complementary sequence is high because of a single-stranded chain to enable the improvement of sensitivity and repeatability to be expected.;COPYRIGHT: (C)2006,JPO&NCIPI