ANTIGEN RAPID DETECTING AND/OR MEASURING METHOD BY FLUORESCENT CORRELATION SPECTRAL METHOD

摘要

PROBLEM TO BE SOLVED: To provide an antigen detecting and/or measuring method capable of rapidly and accurately detecting and/or measuring an antigen such as off-type prion or antigen protein like harmful protein contained in a food material by simple operation.;SOLUTION: In the detection and measurement of an antigen molecule using a fluorescent correlation spectral method (FCS), a fluorescence labelled antibody segment and a non-fluorescence labelled perfect antibody bonded to the fluorescence labelled antibody segment through the antigen are used to produce a significant difference at a diffusion speed between the fluorescence labelled antibody segment not bonded to the antigen and a composite formed by the antigen-antibody reaction of the fluorescence labelled antibody segment-antigen-non-fluorescence labelled perfect antibody and the antigen can be detected and measured using FCS even in the case of an antigen like antigen protein relatively low in its molecular weight without relying on the shape or molecular weight of the antigen to rapidly measure a wide range of an antigen.;COPYRIGHT: (C)2006,JPO&NCIPI