METHOD AND DEVICE CONSTITUTION FOR OPTICAL UNDERSTANDING OF WAVELENGTH-DEPENDENT CHARACTERISTIC VALUES OF ILLUMINATED SAMPLE

摘要

PPROBLEM TO BE SOLVED: To provide a method for optical understanding of the wavelength-dependent characteristic values of an illuminated sample, especially those related to emission and/or absorption behavior, preferably the fluorescence and/or luminescence and/or phosphorescence and/or enzyme-activated light emitting and/or enzyme-activated fluorescence. PSOLUTION: In the method for the optical detection of the wavelength-dependent characteristic values of the illuminated sample, at least one spectral centroid and/or the maximum of emitted radiation and/or absorbed radiation is determined. Furthermore, the centroid and/or the maximum of the emitted radiation of fluorochromes is/are determined for differentiating between different dyes, and/or for determining the local dye composition of a pixel if plural colors are being used simultaneously, and/or for determining the local displacement of the emission spectrum according to the local environment to which the dye is bonded, and/or for determining the ion concentrations based on the emissivity-identifying dyes. PCOPYRIGHT: (C)2006,JPO&NCIPI