The new rearrangement baculovirus, the construction manner and the transcription unit where two below constituent

摘要

(57) Abstract bakiyurouirusu porihedorin (PH), rearrangement bakiyurouirusu which produces the rearrangement polyhedron from the Bacillus thuringiensis crystal protein (CP) and the jellyfish Aequorea victoria green fluorescent protein (GFP) being formed introducing the fusion protein where PH, CP and GFP are directly connected continually from N end to C end into the insect cell simultaneously, with gene introduction vector and wild type bakiyurouirusu which include the fused gene which the cord/code is done is constructed by culturing. In this bakiyurouirusu gene introduction vector pColorBtrus, from plasmid pGFP the DNA fragment which GFP the cord/code is done, from the wild type Autographa californica nuclear polyhedrosis virus the PH gene, synthesizing the Cry1Ac gene from the plasmid pPN6.6 which includes the Bacillus thuringiensis Cry1Ac gene with the respective polymerase chain reaction, in order the DNA fragment which first, the cord/code does GFP 5 '' - end the PH gene 3 '' - to be connected to end, inserting DNA fragment and the PH gene which GFP the cord/code are done to bakiyurouirusu manifestation vector pAcUW31, next, with the PH gene and the DNA fragment which GFP the cord/code is done inserting the Cry1Ac geneDepending, it is constructed. As for the rearrangement virus of this invention, it is possible, to become extinct the noxious insect with the insecticidal activity which is superior among brief in addition because the expedient which the infection insect monitoring is done is had, it is possible to prevent excess use.