Human ADAMTS-1 protein, gene encoding it, pharmaceutical composition, and method for immunological analysis of human ADAMTS-1 protein

摘要

PROBLEM TO BE SOLVED: To obtain the subject new protein, comprising a human ADAMTS-1 protein composed of a specific amino acid sequence, capable of reducing the numbers of leucocytes and blood platelets, increasing the number of erythrocytes and useful for treatment, etc. of rheumatic arthritis, asthma, Kawasaki disease, etc. SOLUTION: This new human ADAMTS-1 protein comprises an amino acid sequence represented by the formula, has actions of reducing the numbers of leucocytes and blood platelets and simultaneously increasing the number of erythrocytes and is effective in treating various inflammatory diseases such as rheumatic arthritis, psoriasis, asthma, hepatitis, Kawasaki disease, gout, adult respiratory distress syndrome, Crohn's disease, ulcerative colitis, sepsis or nephritis, polycythemia rubra, cardiac infarction, cerebral infarction, multiple organ failure, anemia, etc. The protein is obtained by cloning a human renal cDNA with a DNA capable of coding a partial protein of the human ADAMTS-1 as a primer according to a polymerase chain reactional(PCR) method, integrating the resultant human ADAMTS-1 gene into a vector and expressing the gene in Escherichia coli.