首页> 外国专利> Microarray comprising probes for drug-resistant hepatitis b virus detection, quality control and negative control, and method for detecting drug-resistant hepatitis b virus using the same

Microarray comprising probes for drug-resistant hepatitis b virus detection, quality control and negative control, and method for detecting drug-resistant hepatitis b virus using the same

机译:包括用于抗药性乙型肝炎病毒检测,质量控制和阴性对照的探针的微阵列以及使用其的抗药性乙型肝炎病毒检测方法

摘要

Provided are a microarray manufactured using a mixture of target probes for drug-resistant HBV detection, quality control probes for controlling quality in probe hybridization and fabrication of microarrays, and negative control probes for determining the presence and ratio of more than one type, i.e., a wild type and a mutant in a codon, measuring a background of non-specific cross-hybridization, and discriminating homozygotes and heterozygotes, and a method of detecting a drug-resistant HBV, controlling the quality of a microarray, determining the presence and ratio of more than one type, and determining positive and false positive probes at the same time using the microarray. The microarray, which includes the target probes for drug-resistant HBV detection, the QC probes, and the negative control probes, can detect a drug-resistant HBV, control quality in fabrication of microarrays and hybridization, determine the presence and ratio of more than one type, i.e., a wild type and a mutant, determine positive and false positive probes by measuring a background of non-specific cross-hybridization, and discriminate homozygotes and heterozygotes. When a plurality of sets of probes, each set containing target probes, QC probes, and negative control probes, are immobilized on a support of the microarray, detection of resistance in HBV to multiple drugs, quality control, and determination as to the presence and ratio of a wild type and a mutant and whether each probe is positive or false positive can be rapidly and accurately performed.
机译:提供了一种微阵列,该微阵列使用用于抗药性HBV检测的目标探针,用于控制探针杂交和微阵列制造质量的质量控制探针以及用于确定一种以上类型(即,密码子中的野生型和突变体,以测量非特异性交叉杂交的背景并区分纯合子和杂合子,以及检测耐药性HBV,控制微阵列质量,确定其存在和比例的方法多于一种类型,并使用微阵列同时测定阳性和假阳性探针。该微阵列包括用于抗药性HBV检测的目标探针,QC探针和阴性对照探针,可检测抗药性HBV,控制微阵列制造和杂交的质量,确定存在或存在的比例超过一种类型,即野生型和突变型,通过测量非特异性交叉杂交的背景来确定阳性和假阳性探针,并区分纯合子和杂合子。当将多套探针固定在微阵列支持物上时,每套探针均包含靶探针,QC探针和阴性对照探针,检测HBV对多种药物的耐药性,进行质量控制,并确定存在和否。可以快速,准确地检测出野生型与突变体的比率以及每种探针是阳性还是假阳性。

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