Methods and kits for determining ubiquitin protein ligase (E3) activity

摘要

The present invention provides a method and an assay kit for detecting ubiquitin ligase (E3) activity in a sample. The method includes the steps of (1) preparing a sample mixture comprising ubiquitin, a ubiquitin activating enzyme (E1), a ubiquitin conjugating enzyme (E2), a reaction buffer, and the sample; (2) incubating the sample mixture under conditions suitable for ubiquitination to occur; and (3) detecting a presence of ubiquitination products in the sample mixture after the incubation.