Method of quantitating proteins and genes in cells using a combination of immunohistochemistry and in situ hybridization

摘要

The disclosure provides a system for and method of accurately determining the status of a protein target and a nucleic acid target in a biological sample (e.g., a tissue or cell sample). Provided are methods of quantitating a protein target and a nucleic acid target in a tissue or cell sample using image analysis to correlate the optical density of a chromogenic stain to the concentration of a target molecule in a biological sample. The use CISH in combination with IHC to accurately determine the status of two independent target molecules is provided. A computer-aided image analysis system is used to process and analyze optical images of a chromagen-stained tissue or cell sample and to determine the optical density of the stained tissue or cell sample.