The present invention provides a novel reporter gene assay. In particular, the present invention provides a histochemical reporter gene assay employing α-L-arabinofuranosidase in combination with a chromogenic α-L-arabinofuranosidase substrate. The α-L-arabinofuranosidase histochemical reporter assay may be utilized to characterize gene regulatory regions of interest either alone or in combination with other assays.
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机译：本发明提供了一种新颖的报告基因测定。特别地，本发明提供了一种使用α- L -阿拉伯呋喃糖苷酶与生色α- L -阿拉伯呋喃糖苷酶底物组合的组织化学报告基因。 α- L -阿拉伯呋喃糖苷酶组织化学报告基因测定法可单独或与其他测定法结合用于表征目的基因调控区域。
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9. Pim-1 kinase inhibits the activation of reporter gene expression in Elk-1 and c-Fos reporting systems but not the endogenous gene expression: an artifact of the reporter gene assay by transient co-transfection
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B. Yan,H. Wang,T. Kon,2006
