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Application of aspen MADS-box genes to alter reproduction and development in trees

机译:白杨MADS-box基因在改变树木繁殖和发育中的应用

摘要

The present invention provides compositions and methods for producing a transgenic plant that exhibits altered characteristics resulting from over expression or under expression of a novel polypeptide PtM3 or its homolog PtM4. The altered characteristics resulting from over-expression include at least one of the ability to convert axillary meristem to floral meristem; to accelerate flowering i.e., early flowering; to increase fruit production; to increase nut production; to increase seed output; to increase branching; to increase flower production; to increase fruit yield; to increase flower yield and a combination thereof. The altered characteristics resulting from suppressed expression include at least one of complete sterility; partial sterility (sterility of only one sex of a bisexual plant); reduced pollen production; decreased flowering; increased biomass and combinations thereof. Furthermore, once the transgenic plant is sterile, additional exogenous sequences may be incorporated into the sterile plant genome, resulting in other desired plant characteristics. Related promoter, gene constructs, methods, antibodies and kits are also provided.
机译:本发明提供了产生转基因植物的组合物和方法,所述转基因植物由于新多肽PtM3或其同源物PtM4的过表达或表达不足而表现出改变的特性。由过表达引起的改变的特性包括将腋生分生组织转化为花分生组织的能力中的至少一种。加快开花,即早开花;增加水果产量;增加坚果产量;增加种子产量;增加分支;增加鲜花产量;增加水果产量;增加花的产量及其组合。由抑制表达引起的改变的特征包括完全不育中的至少一种;即完全不育。部分不育(双性恋植物仅一种性别的不育);花粉产量减少;开花减少;增加的生物量及其组合。此外,一旦转基因植物是无菌的,就可以将其他外源序列掺入无菌植物基因组中,从而产生其他所需的植物特性。还提供了相关的启动子,基因构建体,方法,抗体和试剂盒。

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