Method for obtaining a botulinum toxin composition comprising a Biologically Active, Clostridium botulinum and a Culture medium to produce botulinum toxin by Said method and a method for preparing a Pharmaceutical composition substantially free of Animal Product in which I

摘要

A method to obtain a Biologically Active botulinum toxin, which comprises the following steps: a) providing a fermentation Medium substantially free of animal derived product; (b) cultivate a botulium Clostridium bacteria in the fermentation Medium under conditions S that allow the production of botulinum toxin A,And (c) to recover a botulinum toxin; Biologically Active Composition of the fermentation Medium. Bolulinum including Clostridium and a Culture medium to produce botulinum toxin where the environment is substantially free of animal derived Product and comprises a product Protein derived from a Plant.A method for preparing a Pharmaceutical composition substantially free of Animal Product in which the active ingredient is a botulinum toxin, which comprises the following steps: a) obtaining a botulinum toxin Biologically Active by the method according to any of L As claims 1 to 9; and (b) Develop botulinum toxin with a suitable excipient.This Way of preparing a Pharmaceutical composition substantially free of Animal Product in which the active ingredient is a botulinum toxin.