Preparation processes of microorganisms evoluu00ecdos and a proteu00ecna evoluu00ecda and biotransformation gene evoluu00ecdo, proteu00ecna evoluu00ecda,And the use of a microorganism or a proteu00ecna evoluu00ecda evoluu00ecdo

摘要

"Preparation processes of micro-organisms evoluu00ecdos and a proteu00ecna evoluu00ecda and biotransformation gene evoluu00ecdo, proteu00ecna evoluu00ecda,And the use of a micro organism evoluu00ecdo or a proteu00ecna evoluu00ecda ".The present invention is a new preparation process of micro organism evolved enabling a modification of metabolic pathways.Characterized by the fact that comprises the following steps: a) obtaining a modified micro organism for genetic modification of cells of a microorganism initial way to inhibit the productionOr the consumption of a metabolite when the organism is grown on a defined,In this way, the ability of affecting growth of micro organism; (b) the culture of microorganisms modified previously obtained on said means set to do it may be necessary to evolve;Add a co substrate to the environment defined to enable this development; (c) the selection of modified micro organism cells able to grow on the environment defined,Perhaps with a co substrate.The invention also concerns of strains of evolved microorganisms thus obtained.The genes encoding for proteins evolved evolved may be obtained by the process according to the invention and use of said micro-organisms.Genes or proteins evolved in a process of biotransformation.