CIRCULAR DNA MOLECULE WITH CONDITIONAL ORIGIN OF REPLICATION WHICH CONTAINS NUCLEIC ACID OF INTEREST AND SELECTION MARKER BUT DOES NOT ENCODE ITS REPLICATION INITIATION PROTEIN, METHOD FOR PREPARING THE SAME AND USE THEREOF IN GENE THERAPY

摘要

A novel circular DNA molecule (I), useful in gene therapy, comprises = 1 sequence of interest, the replication of which is controlled by an origin of replication (ORI) from a plasmid or bacteriophage, which is only active in a host cell in the presence of one or more specific proteins foreign to that cell.