TETANUS TOXIN FUNCTIONAL FRAGMENT ANTIGEN AND TETANUS VACCINE.

摘要

TETANUS TOXIN FUNCTIONAL FRAGMENT ANTIGEN, COMPRISING AT LEAST ONE FRAGMENT WHICH IS THE SAME AS THAT OBTAINABLE BY A PROCESS COMPRISING THE STEPS OF SPLITTING AT LEAST ONE PEPTIDE BOND SELECTED FROM PEPTIDE BONDS INDIVIDUALLY CONNECTING MUTUALLY ADJACENT AMINO ACID RESIDUES IN A PARTIAL AMINO ACID SEQUENCES BETWEEN TOW CYSTEINE RESIDUES PARTICIPATING IN FORMING A DISULFIDE BRIDGE PRESENT IN THE N-TERMINAL OF THE ENTIRE AMINO ACID SEQUENCE OF THE WHLE TETANUS TOXIN MOLECULE SHOWN IN SEQ ID NO:1, SPLITTING SAID DISULFIDE BRIDGE, AND SPLITTING NON-COVALENT BONDS BETWEEN GROUPS ON THE TETANUS TOXIN MOLECULE; SAID TETANUS TOXIN FUNCTIONAL FRAGMENT ANTIGEN HAVING: (a) A MOLECULAR WEIGHT OF FROM 90,000 TO 110,000 AS MEASURED BY AN SDS-POLYACRYLAMIDE GEL ELECTROPHORESIS METHOD; (b)AN ISOELECTRIC POINT OF 7.25 + 0.5 AS MEASURED BY AN ISOELECTRIC FOCUSING METHOD; AND (c)AN IMMUNOPOTENCY WHICH IS THE SAME AS THAT OF A WHOLE TETANUS TOXIN TOXOID, WHEREIN EACH OF SAID AT LEAST ONE FRAGMENT INDEPENDENTLY HAS AN N-TERIMINAL AMINO ADID SEQUENCE SELECTED FROM THE GROUP CONSISTING OF SEQ ID NO.2, SEQ ID NO.3,SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, SEQ ID NO.8 AND SEQ ID NO.9.