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Identification of antigenically important neisseria antigens by screening insertional mutant libraries with antiserum
Identification of antigenically important neisseria antigens by screening insertional mutant libraries with antiserum
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机译:通过用抗血清筛选插入突变体文库来鉴定具有重要抗原意义的奈瑟菌抗原
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摘要
A method for identifying a polypeptide of a microorganism which polypeptide is associated with an immune response in an animal which has been subjected to the microorganism, the method comprising the steps of (1) providing a plurality of different mutants of the microorganism; (2) contacting the plurality of mutant microorganisms with antibodies from an animal which has raised an immune response to the microorganism or a part thereof, under conditions whereby if the antibodies bind to the mutant microorganism the mutant microorganism is killed; (3) selecting surviving mutant microorganisms from step (2); (4) identifying the gene containing the mutation in any surviving mutant microorganism; and (5) identifying the polypeptide encoded by the gene. The polypeptide identified or a variant or fragment thereof or a fusion of these is useful in a vaccine. The polypeptide may be a polypeptide comprising the amino acid sequence selected from any one of SEQ ID Nos 2, 4, 6, 8, 10, 12, 14, 16, 18, 25 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56; or a fragment or variant thereof or a fusion of such a fragment or variant, and is useful in a vaccine against Neisseria meningitidis.
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机译:一种鉴定微生物多肽的方法,该多肽与已经受到该微生物的动物中的免疫反应有关,该方法包括以下步骤:(1)提供多种不同的微生物突变体; (2)使多种突变微生物与来自对微生物或其部分产生免疫反应的动物的抗体接触,条件是如果抗体结合突变微生物,则杀死突变微生物; (3)从步骤(2)中选择存活的突变微生物; (4)在任何存活的突变微生物中鉴定包含该突变的基因; (5)鉴定该基因编码的多肽。鉴定的多肽或其变体或片段或它们的融合体可用于疫苗。多肽可以是包含选自SEQ ID Nos 2、4、6、8、10、12、14、16、18、25 20、22、24、26、28、30, 32、34、36、38、40、42、44、46、48、50、52、54、56;或其片段或变体或此类片段或变体的融合体,可用于抗脑膜炎奈瑟氏球菌的疫苗。
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