LIPASE VARIABLES STIMULATED BY BILIARY SALTS, DNA MOLECULES THAT CODE THEM AND NOT TRANSGENIC HUMAN MAMMALS

摘要

The invention discloses nucleic acids encoding variant Bile Salt Stimulated Lipase (BSSL; EC 3.1.1.1). The encoded variant BSSL enzymes maintain catalytic activity but contain fewer glycosylation sites that full-length BSSL. This reduced glycosylation facilitates purification and characterization of recombinant BSSL proteins.