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METHODS FOR ESTEOPOROSEDIAGNOSIS AND FOR TESTING POTENTIAL OSTEOPOROSIS THERAPEUTIC USE OF STANDARDIZED, PRIMARY OSTEOBLASTIC CELL CULTURES FROM OSTEOPOROTIC PATIENTS
METHODS FOR ESTEOPOROSEDIAGNOSIS AND FOR TESTING POTENTIAL OSTEOPOROSIS THERAPEUTIC USE OF STANDARDIZED, PRIMARY OSTEOBLASTIC CELL CULTURES FROM OSTEOPOROTIC PATIENTS
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机译:骨质疏松患者标准化,原代成骨细胞培养物的骨质疏松诊断方法和潜在的骨质疏松治疗方法
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摘要
A method of screening potential therapeutic substances that may be useful in the treatment of osteoporosis is provided herein utilizing primary osteoblast precursor cell cultures to determine the mitogenic effect of the tested agents. This method comprises preparation of the primary cultures from osteoporotic patients for the expression of several intracellular proteins in early and late osteoblast differentiation phases, expression of other proteins involved in matrix synthesis, and quantitative analysis of the cellular proliferation rate. The expression of such intracellular proteins and cell proliferation in the cultures derived from osteoporotic patients are quantitatively compared to primary osteoblast cell cultures from non-osteoporotic patients that are standardized for the same approximate age and sex.
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