annexin proteins and auto antibodies as serummarker for lung cancer and u00f6sophaguskrebs

• 1. 肺癌血清诊断标志物及其应用、肺癌相关可溶性蛋白的分离鉴定方法 [P] . 中国专利： CN110244047A . 2019-09-17
• 2. 血清蛋白标志物组合在肺癌筛查和诊治中的应用 [P] . 中国专利： CN109085355A . 2018-12-25
• 3. annexin proteins and auto antibodies as serummarker for lung cancer and u00f6sophaguskrebs [P] . 德国专利： DE60028040D1 . 2006-06-22

  机译： Annexin蛋白和自身抗体可作为肺癌和食道癌的血清标志物

• 4. Annexin - proteins and auto - antibody as markers for lung cancer and oesophageal cancer [P] . 德国专利： DE60028040T2 . 2006-11-23

  机译： Annexin蛋白和自身抗体作为肺癌和食道癌的标记

• 5. auto antibodies against proteins as markers for lung cancer annexin [P] . 德国专利： DE60041839D1 . 2009-04-30

  机译： 蛋白自身抗体作为肺癌膜联蛋白的标志物

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• 4. Effect of the adaptive intermittent ventilation before radical operation for lung cancer under one-lung ventilation on the non-ventilated lung tissue injury and apoptosis molecule protein expression [J] . Xue-Mei Yang 海南医科大学学报(英文版) . 2017,第018期
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• 6. Association of genetic determinants of alpha one antitrypsin, neutrophil elastase, and matrix metalloproteinase one with the risk of COPD, lung cancer, and lung cancer survival. [D] . Enewold, Lindsey R. 2008
• 7. Lung cancer-associated auto-antibodies measured using seven amino acid peptides in a diagnostic blood test for lung cancer [O] . Nada H Khattar, Sarah P Coe-Atkinson, Arnold J Stromberg, 2010
• 8. Lung cancer-associated auto-antibodies measured using seven amino acid peptides in a diagnostic blood test for lung cancer [O] . Khattar, Nada H, Coe-Atkinson, Sarah P, Stromberg, Arnold J, 2010

• 1. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 2. 血清蛋白质组学技术在筛选胃癌血清蛋白质标志物中的应用 [C] . 刘文韬 ,刘炳亚 ,辛林 . 第四届中国肿瘤学术大会暨第五届海峡两岸肿瘤学术会议 . 2006
• 3. 应用蛋白芯片技术筛选肺癌和肺癌骨转移患者的血清标志物并探讨其表达意义 [A] . 曹亚男 . 2019