Improved production of taxanes by cell cultures of Taxus species.

摘要

The invention provides methods by which CAN PRODUCE taxol, baccatin II, AND OTHER COMPOUNDS OF TYPE TAXOL, or taxanes, with a very high yield, FROM ALL KNOWN Taxus species, eg BREVIFOLIA, canadensis, cuspidata Baccata, GLOBOSA, floridana, wallichiana, MEDIA AND CHINENSIS. It has been found that the specific culture conditions MODIFICATIONS (IE COMPOSITIONS MEDIA AND MODES) increase the performance of various taxanes from cell culture of all species of Taxus. Especially preferred agents INCLUDE complex or silver ion, jasmonic acid (especially the methyl ester), REGULATORS auxin-related growth, and inhibitors of phenylpropanoid metabolic pathway, such as acid 3,4 - methylenedioxy - 6 - nitrocinnamic. Enhancers such agents may be used alone or in combination with other conditions WHETHER OR THE RENDIMIENTO.SI-increasing PERFORMANCE taxanes from cell culture of T. chinensis PLANT INCREASES especially through use of one or more such conditions, EQUALLY has been found that the yield of taxanes for all species Taxus iMPROVEMENT WITH THE USE OF SUCH CONDITIONS.