An enzymatic process to obtain 4-0-bd-galactopyranosyl-D-xylose, 4-OBD-galactopyranosyl-D-xylose obtained according to the procedure, the continenen compositions and their use in the evaluation of intestinal lactase.

摘要

A galactopyranosyl-D-xylose 4-0 -D-, useful in the in vivo evaluation of intestinal lactase activity in humans enzymatic process to obtain, comprising: a) reacting for 2-48 h, D-xylose and aD galactopyranoside in a buffer at pH 5.0-9.0 and at a temperature ranging from the freezing point of the mixture and 45 ° C, which 10-10,000 units aD-galactosidase are added per gram of aD-galactopyranoside medium; b) stopping the reaction by deactivation of the enzyme and c) isolating and crystallizing fractions containing 4--D-galactopyranosyl-D-xylose in a crystallization mixture selected between acetonal / methanol (5: 1 to 20: 1) and acetone / water (5: 1 to 20: 1)