PROCESS FOR THE PURIFICATION OF HUMAN INTERLEUKIN-1 RECEPTOR ANTAGONIST FROM RECOMBINANT E. COLI

摘要

A process for purifying human Interleukin-1 receptor antagonist (IL-1ra),obtained by fermenting a strain of recombinant E. coli,which comprises: a) loading the mixture to be purified, which has beenbuffered at a pH value lower than 6.2 and optionally diluted toreduce its ionic strength at a low value, onto a cationic exchange matrix andeluting said matrix with an aqueous buffered solution having apH value from about 7.5 to 9.0 and a low ionic strength; and b) applying theeluate from the cationic exchange step, containing the desiredIL-1ra protein, directly onto an anionic exchange matrix and eluting with anaqueous buffered solution having a pH value within the aboverange 7.5 to 9.0 and an increased ionic strength.