ASSAYS FOR AGENTS WITH SELECTIVE CYTOTOXICTY TO HDAC RESISTANT CELL LINES

摘要

This invention relates to a method of identifying and/or obtaining an agentwhich is selectively cytotoxic to histone deacetylase inhibitor (HDACi)resistant cells, said method comprising the steps of: a) incubating HDACiresistant cells and HDACi sensitive cells with a test agent, b) determiningthe cytotoxicity of the test agent for said HDACi resistant cells and thecytotoxicity of the test agent for said HDACi sensitive cells, wherein anincrease in cytoxicity of the test agent for said HDACi resistant cellsrelative to said HDACi sensitive cells is indicative that the test agent is anagent which is selectively cytotoxic to HDACi resistant cells. The inventionis based on the recognition that cells which are resistant to inhibitors ofhistone deacetylases (HDACis) such as PXD101 are hypersensitized to certainanti-cancer agents.