Assays for agents with selective cytotoxicty to HDAC resistant cell lines

摘要

This invention relates to a method of identifying and/or obtaining an agent which is selectively cytotoxic to histone deacetylase inhibitor (HDACi) resistant cells, said method comprising the steps of: a) incubating HDACi resistant cells and HDACi sensitive cells with a test agent, b) determining the cytotoxicity of the test agent for said HDACi resistant cells and the cytotoxicity of the test agent for said HDACi sensitive cells, wherein an increase in cytoxicity of the test agent for said HDACi resistant cells relative to said HDACi sensitive cells is indicative that the test agent is an agent which is selectively cytotoxic to HDACi resistant cells. The invention is based on the recognition that cells which are resistant to inhibitors of histone deacetylases (HDACis) such as PXD101 are hypersensitized to certain anti-cancer agents.