Method for pH-biased isoelectric trapping separations

摘要

A method for altering the initial composition of an ampholytic component containing sample by isoelectric trapping provides for selecting a first isoelectric buffer having a pI value different from the pI value of a first ampholytic sample component in the sample, contacting the isoelectric buffer with the first ampholytic sample component, and obtaining the first ampholytic sample component in a non-isoelectric state on one side of an isoelectric separation barrier at the end of an isoelectric trapping process.