METHODS AND COMPOSITIONS FOR IDENTIFYING TARGET CELL CYTOLYTIC LYMPHOCYTES IN A SAMPLE

摘要

Methods and compositions for identifying target cell cytolytic lymphocytes, e.g., T-cells, such as neoplastic cell cytolytic T-cells, in a subject are provided. In practicing the subject methods, the sample is contacted with a target cell stimulator, e.g., a neoplastic cell, and a detectably labeled granule membrane protein specific binding agent. Following contact, any resultant labeled lymphocytes, e.g., T-cells, are identified as lymphocytes cytolytic for the target cell. Also provided are compositions, kits, and systems for practicing the subject methods. The subject methods find use in a variety of different applications, including disease/therapy monitoring applications and therapeutic applications.