HCTLA4-IG FUSION PROTEIN-CODING GENE, RECOMBINANT VECTOR COMPRISING THE SAME, AND PROCESS FOR PRODUCING HCTLA4-IG FUSION PROTEIN USING THE SAME

摘要

Provided are a gene coding for a human cytotoxic T lymphocyte antigen 4-Immunoglobulin (hCTLA4-Ig) fusion protein comprising a plant-derived leader sequence, and recombinant vectors pMYN409, pMYN413 and pMYN414, comprising the same. Provided are also host cells transformed with the above-mentioned recombinant vectors, a process for producing a recombinant hCTLA4-Ig fusion protein, involving suspension culturing the host cells, and an hCTLA4-Ig fusion protein prepared using the same. In accordance with the present invention, it is possible to achieve mass production of the recombinant hCTLA4-Ig fusion protein having immunosuppressive activity equal to that of the hCTLA4-Ig fusion protein expressed in conventional animal cells, by use of plant cell suspension culture. Thereby, recombinant proteins can be produced more efficiently and economically, as compared to animal cell culture techniques.