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FLUORESCENCE POLARIZATION-BASED HOMOGENEOUS ASSAY FOR FUMONISIN DETERMINATION IN GRAINS
FLUORESCENCE POLARIZATION-BASED HOMOGENEOUS ASSAY FOR FUMONISIN DETERMINATION IN GRAINS
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机译:基于荧光偏振的均质测定谷物中伏马毒素的方法
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摘要
A homogeneous assay for determining the fumonisin content in grains uses the technique of fluorescence polarization. A grain extract is prepared by shaking a crushed grain sample with a solvent. A mixture is prepared by combining the grain extract with a tracer and with monoclonal antibodies specific to fumonisin. The tracer is able to bind to the monoclonal antibodies to produce a detectable change in fluorescence polarization. The tracer is prepared by conjugating fumonisin to a suitable fluorophore. The fluorescence polarization of the mixture is measured. The fumonisin concentration of the mixture may be calculated using a standard curve obtained by measuring the fluorescence polarization of a series of fumonisin solutions of known concentration.
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