METHODS, SYSTEMS AND COMPUTER READABLE MEDIA FOR IDENTIFYING DYE-NORMALIZATION PROBES

摘要

Methods, systems and computer readable media for identifying dye-normalization probes. Intensity signals read from probes on a set of existing multi-channel microarrays are provided. The intensity signals are combined from each channel for each probe to generate a combined signal intensity value for each probe on each array. For each probe, the combined signal intensity values are combined across all arrays to provide an ordered sequence of probes from a lowest overall signal to a highest overall signal. The probes are then ranked according to the results of combining the combined signal intensity values, and binned into a plurality of bins. With regard to each probe, a metric representative of the multi-array distance of the signal intensities of the probe from a neutral expression value across all arrays is calculated and the probes are ranked within each bin based on the calculated metrics. Candidate dye-normalization probes may be selected by selecting at least one of the lowest ranked probes within each bin. Optionally, at least one of the lowest ranked probes in each bin may be discarded as outliers, and then at least one of the remaining lowest ranked probes may be selected from each bin as the candidate dye-normalization probes.