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METHOD TO DETECT GENE POLYMORPHISMS AND MONITOR ALLELIC EXPRESSION EMPLOYING A PROBE ARRAY
METHOD TO DETECT GENE POLYMORPHISMS AND MONITOR ALLELIC EXPRESSION EMPLOYING A PROBE ARRAY
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机译:探针阵列检测基因多态性和监测等位基因表达的方法
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摘要
The invention provides methods of monitoring expression levels of different polymorphic forms of a gene. Such methods entail analyzing genomic DNA from an individual to determine the presence of heterozygous polymorphic forms at a polymorphic site within a transcribed sequence of a gene of interest. RNA from a tissue of the individual in which the gene is expressed is then analyzed to determine relative proportions of polymorphic forms in transcript of the gene. Having identified alleles of a gene that are expressed at different levels, the alleles can be further analyzed to locate a second polymorphism that has a causative role in the different expression levels. The methods are amenable to analyzing large collections of genes simultaneously using arrays of immobilized probes.
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