THERMOSTABLE ENZYME PROMOTING THE FIDELITY OF THERMOSTABLE DNA POLYMERASES- FOR IMPROVEMENT OF NUCLEIC ACID SYNTHESIS AND AMPLIFICATION IN VITRO

摘要

A purified thermostable enzyme is derived from the thermophilic archaebacterium iArchaeoglobus fulgidus/i. The enzyme can be native or recombinant, is stable under PCR conditions and exhibits double strand specific exonuclease activity. It is a 3'-5' exonuclease and cleaves to produce 5'-mononucleotides. Thermostable exonucleases are useful in many recombinant DNA techniques, in combination with a thermostable DNA polymerase like iTag/i especially for nucleic acid amplification by the polymerase chain reaction (PCR).