KIT FOR IN PROCESS QUALITY CONTROL TO PREDICT AND DETERMINE ENGRAFTMENT AND MULTILINEAGE RECONSTITUTION POTENTIAL OF HEMATOPOIETIC CELLS AND CORD BLOOD STORAGE

摘要

The present invention relates generally to kits that provide reagent mixes and instructions for the use thereof, in performing high-throughput assay methods for stem cell transplantation and cord blood storage quality control and methods of use. The methods measure the luminescent output derived from the intracellular ATP content of incubated target cells, and correlate the luminescence with the proliferative status of the cells. By non-subjectively detecting and quantitatively measuring the proliferation potential of several hematopoietic stem and progenitor cell populations simultaneously in a prospective or even retrospective manner, the present invention can determine the 'quality', engraftment, repopulation and multilineage reconstitution potential of the infused cells, thereby significantly increasing the ability to predict the transplant outcome and reducing risk to the patient.