Instrument and methods for analysing a multiplicity of fluorescent dyes using a multiplicity of photodetectors having adjustable gains

摘要

An instrument for analysing a multiplicity of fluorescent dyes is disclosed, said instrument comprising a multiplicity of photodetectors, each having an adjustable gain, and a stored representation of pairwise functional relationships between measured fluorescence and photodetector gain for each of said photodetectors and for each of said fluorescent dyes. Preferably, the instrument is a flow cytometer. Furthermore, methods are disclosed for calculating compensation/quantitation for arbitrary sets of photodetector gain settings which do not require the analysis of additional setup samples.