PROCESS FOR PRODUCTION OF CHICKEN RECOMBINANT DIVALENT ANTIBODY FROM CHICKEN SINGLE-CHAIN VARIABLE FRAGMENT (scFv) AND ANTIBODY PRODUCED BY THE PROCESS

摘要

Disclosed is a process for the simple production of a chicken recombinant divalent antibody by dimerizing a single-chain variable fragment (scFv) produced by phage display method to produce the chicken antibody. Also disclosed is an antibody produced by the process. Further discloses is a representative application of the antibody. A process for producing a chicken recombinant divalent antibody comprising the steps of amplifying a light-chain variable region gene and a heavy-chain variable region gene using, as a template, a polynucleotide encoding a chicken single-chain variable fragment, preparing a gene fragment for the expression of a light chain by ligating a light-chain leader sequence operable in a host cell, the light-chain variable region gene and a gene for a light-chain constant region of a chicken antibody, and preparing a gene fragment for the expression of a heavy chain by ligating a heavy-chain leader sequence operable in the host cell, the heavy-chain variable region gene and a gene for a heavy-chain constant region of the chicken antibody.