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METHOD OF SEPARATING SUGAR FROM COMPOUND HAVING GLYCOSIDE BOND, SUGAR SEPARATION SYSTEM, SUGAR SEPARATION AGENT KIT, STANDARDIZED SAMPLE FOR SUGAR SEPARATION AND ASSESSMENT SYSTEM
METHOD OF SEPARATING SUGAR FROM COMPOUND HAVING GLYCOSIDE BOND, SUGAR SEPARATION SYSTEM, SUGAR SEPARATION AGENT KIT, STANDARDIZED SAMPLE FOR SUGAR SEPARATION AND ASSESSMENT SYSTEM
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机译:从具有糖苷键的复合物中分离糖的方法,糖分离系统,糖分离剂套件,用于糖分离和评估系统的标准样品
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摘要
In an in-flow chemical reaction system (A) of the present invention, a sample solution (1) containing glycoprotein is injected into a reaction tube (7) in which an alkaline solution continuously flows. When a mixed solution of the sample solution and the alkaline solution reach a thermostat bath (6), reaction for cleaving a glycoside linkage takes place, thereby separating a carbohydrate chain from glycoprotein. From the solution containing the carbohydrate chain, alkali is removed by using an ion-exchange column (12), so that a fraction of a carbohydrate chain (14) is collected into a sample tube (13). In the in-flow chemical reaction system (A), reaction takes place in minutes, so that reaction time is significantly reduced compared to a conventional method. Thus, a glycoside linkage is readily cleaved with high precision in a short time, thereby separating carbohydrate from a glycoside-linkage-having compound.
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