DIGITAL IMAGING OF SINGLE RADIAL IMMUNODIFFUSION ASSAYS

摘要

A method for performing a single radial immunodiffusion (SRID) assay for an antigen or antibody of interest, comprising the steps of: (a) applying one or more test sample(s) to a gel, wherein the gel contains (i) antibody specific to the antigen of interest and/or (ii) antigen recognised by the antibody of interest; (b) allowing the sample(s) to diffuse radially from its/their point(s) of application; (c) preparing a digital image of the gel after diffusion has taken place; (d) determining the external diameter(s) of precipitation halos within the digital image; (e) comparing the determined diameters to those resulting from one or more standard sample(s) containing a known concentration of the antigen or antibody of interest; and, optionally, (f) using the results from step (e) to determine the concentration(s) of the antigen or antibody of interest in the test sample(s).