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DEVELOPMENT OF MONOCLONAL ANTI-GLYCOHEMOGLOBIN ANTIBODY AND PURIFICATION METHOD OF THE MONOCLONAL ANTIBODY
DEVELOPMENT OF MONOCLONAL ANTI-GLYCOHEMOGLOBIN ANTIBODY AND PURIFICATION METHOD OF THE MONOCLONAL ANTIBODY
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机译:单克隆抗体-糖化血红蛋白抗体的开发及纯化方法
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摘要
The present invention relates to a method of diagnosing diabetes in one of the typical adult diseases, and more particularly relates to the development of monoclonal antibodies for measuring glycated hemoglobin in the diabetic labeling substance present in the blood. The method for measuring glycated hemoglobin in the blood may be identified in three ways. First is ion-exchange chromatography method using a pre-off that occurs when the sugar bound to hemoglobin, and the second method of using a boronate to recognize that the material bound to the cis-diol per hemoglobin, and using the antigen-antibody reaction in the third The immunoassays. In order to produce the immunoassay is essential to develop a specific antibody recognizing the hemoglobin bound to the sugar, and we developed a cell line that produces a unique antibody to glycated hemoglobin using a cell fusion technique in the present invention, by culturing the cell line the antibody was purified using antibody affinity chromatography. The antibody was purified using a immunoassay that its potency is excellent, and the bonded material was confirmed that color development can be measured in the blood glycated hemoglobin selectively.
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