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Real-time analysis of mass spectrometry data for identifying peptidic data of interest
Real-time analysis of mass spectrometry data for identifying peptidic data of interest
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机译:实时分析质谱数据以识别目标肽数据
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摘要
A mass spectrometry system (100) is described. The mass spectrometry system (100) comprises: (a) a mass spectrometer (102); and (b) a controller (104) connected to the mass spectrometer (102). The controller (104)is configured to: (i) direct the mass spectrometer (102) to acquire a precursor ion spectrum of a sample stream (116); (ii) analyze, in real-time, the precursor ion spectrum to determine whether a first evaluation criterion is satisfied; (iii) if the first evaluation criterion is satisfied, direct the mass spectrometer (102) to acquire a product ion spectrum of the sample stream (116); (iv) analyze, in real-time, the product ion spectrum to determine whether a second evaluation criterion is satisfied; and (v) if the second evaluation criterion is satisfied, analyze the product ion spectrum to assign an identification to the product ion spectrum. For certain implementations, the controller (104) allows automated, data-dependent acquisition of mass spectrometry data to improve the efficiency at which peptidic data of interest can be acquired.
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