DEVELOPMENT OF MOLECULAR MARKERS FOR MILK PRODUCTION

摘要

The present invention is a molecular marker of beef mtDNA D-loop region nucleotide variation by the effect on milk production It is to develop and take advantage of them to improve beef. Substitution of a single base in the beef of mtDNA D-looP region was found in 20 regions. Among them, 8169, 16 042, 16 051, 16 057, 16 093, 16 119, 16 122, 16 209, 16 255, a high frequency of nucleotide substitutions were detected in the 16302 bp region. Milk production effect due to base substitution at 16185 bp region is 3.557 (p 0.05) was found to frequency G is substituted with A is found to be 0.063. The nucleotide sequence of which is confirmed by the present invention beef Dayu system mtDNA mutations within the D-loop region of the frequency groups can be divided clearly the differences between the groups as compared to the frequency of the frequency group and the other beef cow group. You can also get a great effect to improve milk production over the beef breeding strategies using molecular genetic techniques prescribed variations of the 16185 bp region of molecular markers.