QUALITATIVE AND QUANTITATIVE DETECTION METHODS USING SPECIFIC PRIMERS, PROBES AND STANDARD PLASMID FOR GM CORNS

摘要

The present invention all of the gene of interest in whole or in part with other expression control region DNA region of the free relates to a qualitative and quantitative analysis of specific primers, probes, and test method using the standard plasmid with them for about genetically modified agricultural products, in particular in the foreign or to a method that can be detected by determining a recombinant gene in various strains by some primers to amplify the region containing the (primer), using the probe (probe) and the standard plasmid (plasmid standard). Genetically modified agricultural PCR (polymerase chain reaction) primers and probes and standard plasmid for black of the present invention are GMO (Genetically Modified Organism) can be commercialized test kits, especially genetically modified corn used for distribution busy or imported agricultural products and food of it can be used for qualitative and quantitative analysis. ; Genetically modified corn, PCR, real time PCR, specific primers, probes, GMO black, cry1Fa2 gene, ubiquitin promoter, CP4-EPSPS gene